Biochemical studies on sphingolipids of Artemia franciscana: complex neutral glycosphingolipids

Artemia franciscana

Artemia franciscana C. Drewes (updated, 2002) http://www.zool.iastate.edu/~c_drewes/ http://www.zool.iastate.edu/~c_drewes/Artemph.jpg Taxonomy Phylum: Arthropoda Subphylum): Crustacea Class: Branchiopoda (includes fairy shrimp, brine shrimp, daphnia, clam shrimp, tadpole shrimp) Order: Anostraca (brine shrimp and fairy shrimp) Genus and species: Artemia franciscana (= the North American versio...

Alternative Biotest on Artemia franciscana

Recent toxicology, in accordance with recommendations from the European Council, has demanded decrease in the number of vertebrates used in toxicology testing and their partial replacement with invertebrate animals, plants or even organ, tissue, or cell cultures. During the last 50 years various invertebrate species have been tested for their sensitivity to many chemical or physical agents to p...

Metabolic downregulation during diapause in embryos of Artemia franciscana

Simultaneous quantification of lyso-neutral glycosphingolipids and neutral glycosphingolipids by N-acetylation with [3H]acetic anhydride.

We describe a new method that permits quantification in the pmol to nmol range of three lyso-neutral glycosphingolipids (lyso-n-GSLs), glucosylsphingosine (GlcSph), galactosylsphingosine (GalSph), and lactosylsphingosine, in the same sample as neutral glycosphingolipids (n-GSLs). Lyso-n-GSLs and n-GSLs are initially obtained from a crude lipid extract using Sephadex G25 chromatography, followed...

Neutral glycosphingolipids of human acute leukemias.

Neutral glycosphingolipids were isolated from the malignant cells of several patients with different types of acute leukemia. Analyses were performed by high performance liquid chromatography combined with enzymatic hydrolysis of glycosphingolipids using glycosidases (Escherichia freundii endo-beta-galactosidase, jack bean beta-galactosidase, and beef kidney beta-hexosaminidase). We found that ...